Journal: bioRxiv
Article Title: APOBEC3A is the predominant global editor of cytosines in human mRNAs and in single-strand RNA viruses
doi: 10.64898/2026.02.10.705151
Figure Lengend Snippet: (A) Enrichment of the 16 possible C to U mRNA editing trinucleotide motifs in human BT-474 breast cancer cell lines expressing APOBEC3A (left panel) or APOBEC3B (right panel). mRNA editing motifs and statistical evaluations are presented in the same format as in for DNA editing motifs. Enrichment values shown on each panel can be found in S3 Table. Red dashed lines indicate enrichment level of 1.0. Black bars indicating a statistically significant enrichment (q < 0.05 determined for 16 P-values by Benjamini-Hochberg); grey bars – not significant. Enrichments for all possible trinucleotide mRNA editing motifs, including P-values and q-values can be found in S7 Table. Pairs of odds ratios of all motif-motif and cohort-cohort combinations (APOBEC3A vs APOBEC3B) within a four-motif uCn group were compared by Breslow-Day Test for homogeneity of the odds ratios (S5 Table). ( B ) The density of APOBEC uCn mRNA editing motif in different secondary structure context in cell lines expressing APOBEC3A (A3A), and APOBEC3B (A3B). ( C ) Densities of each of the four APOBEC3A mRNA editing motifs belonging to the four-motif uCn group in each type of secondary structure. ( B,C ) Brackets show significant differences between densities calculated by chi-square test. Source data for panels B and C and statistical comparisons can be found in S8 Table.
Article Snippet: BT474 cells were obtained directly from ATCC as part of a breast cancer cell line panel, ATCC 30-4500K.
Techniques: Expressing